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April 26, 2018

恭喜徐继坤博士在Scientific Reports发表学术论文

恭喜徐继坤博士Scientific Reports发表论文:

Optimized microwave extraction,characterization and antioxidant capacity of biological polysaccharides from Eucommia ulmoides Oliver leaf,Jikun Xu, Huijie Hou, Jingping Hu, Bingchuan Liu, Scientific Reports (2018) 8:6561


全文:

https://doi.org/10.1038/s41598-018-24957-0


摘要:

Microwave-induced technique was combined with response surface methodology for optimizing the isolation of polysaccharides from Eucommia ulmoides Oliver leaf. The maximum polysaccharides yield of 12.31% was achieved by microwave extraction at 74 °C for 15 min with a solid to liquid ratio of 1:29 g/mL, which agreed with the predicted value and was 2.9-fold higher than that of the conventional heat-reflux extraction method. The dominant bioactive constituent in extracts was chlorogenic acid (1.3–1.9%), followed by geniposidic acid (1.0–1.7%). The polysaccharides from the optimized extraction had a high molecular weight and polydispersity (Mw 38,830 g/mol, Mw/Mn 2.19), as compared to the fraction prepared in the absence of microwave (Mw 12,055 g/mol, Mw/Mn 1.26). Glucose was the dominant sugar component (38.2–39.1%) of heterogeneous polysaccharides which belonged to a structure of β-type acidic heteropolysaccharides with a glucan group and highly branched degree. The polysaccharides showed a higher DPPH radical scavenging index (0.87–1.22) than BHT (0.41) but lower than BHA (3.56), which can act as a favorable antioxidant in functional food.